Electron-Microscopes
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Enormous Mushrooms
I found a stand of white toadstools in a yard behind a shop I frequent. I had not seen mushrooms that big in quite a while. In my younger years I was quite a mushroom enthusiast, not for eating them but for finding and identifying them. September is a good time for mushrooms and if it's wet you can find "blooms" of them under trees or in moist areas.

I believe that these mushrooms are Chlorophylla molybdites which is a common species in this area. They are poisonous. If you are unfortunate enough to eat one, you probably won't die but you will be very sick.
I would count myself as a "nature-lover" but not in the romantic sense. I enjoy being out in "natural" environments so that I can analyze and identify species and think about the ecosystem. I think of all the many species of plants and fungi and animals and birds and insects as a vast problem set waiting for me to identify them and find their place in the system. Or else I am there to make sketches, which are also like problems to be solved. My moments of nature bliss or sense of wonder are less frequent than the enjoyment of having identified a bird or mushroom species, or matching a specific shade of sunlit green under the rustling romantic canopy.
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High-pressure freezing (HPF) has been generally accepted as the most reliable method for cryofixation of biological samples, yielding a deep vitreous freezing. In recent cell biology, mammalian cultured cells are widely used, but HPF of cultured cell monolayer has not reached its full potential. In this study, we developed a new reliable device for HPF of cultured cell monolayer by using a 10-µm-thin stainless disc both as culture plate and specimen carrier. We describe the practical procedure, and demonstrate fine structures of HeLa cells cultured and cryofixed on the stainless discs as results. |
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